Cannabis Terpenes Testing Equipment

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Refurbished HP Diffusion Pump GC/MS System

Refurbished Equipment for Terpenes Analysis

Mass Selective Detector Options:

Hewlett Packard and Agilent - Models 5973, 5973N and 5975C Mass Selective Detectors
Hewlett Packard and Agilent - Model 6890, 6890N and 7890 Gas Chromatograph Mainframes
Hewlett Packard and Agilent Technologies - Split/Splitless inlet with EPC

Used Hewlett Packard 5973 GCMS Systems are offered with optional:

Liquid Autosampler Options:

Hewlett Packard - Model G1512A Autosampler Control Module
Single Hewlett Packard - Model G1513A Autosampler Injector - (7673C Tower for the 6890 GC)
Hewlett Packard - 7673C Tray (18596C)

Agilent 7683 Liquid Autosampler with 100 position sampler tray

Rebuilt HP 5973 Diffusion Pump GCMS Systems will also include the following Chemstation Data System for instrument control and data acquisition.

Computer - Intel Pentium Dual-core 3 MB Cache G20303 GHz Processor, 24X DVD Burner, SATA Dual WD Caviar Blue 500GB Internal SATA Hard Drive, 2GBRAM - Brand New
Agilent - 82350 PCI HPIB Card
Microsoft - Windows XP License
Agilent Technologies - G1701DA ChemStation
Monitor - 19 inch Flat Panel Display 1280 x 1024 (NEW)

Other options are available upon request including: additional injection ports, detectors and autosampler configurations.

Our website is intended to be a catalog of system configurations based on our common inventory items. We can custom configure any system to meet your specific requirements.

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GC/MS Systems for Cannabis Terpenes

The HP and Agilent Mass Selective Detectors are a transmission quadrupole mass spectrometer for qualitative and quantitative analysis by electron ionization (EI), The GCMS can be configured with additional accessories to adapt them for various applications. The MS Productivity ChemStation Data System can process data from both the MS and conventional GC detectors. Below is a partial list of options that may be added to enhance the sample introduction, separation, and detection capabilities.

Cannabis Terpenes

Currently Terpenes are being studied and tested as anticancer agents in clinical trials, and have shown particular success in treating liver cancer. In clinical studies, they've been shown to selectively kill tumor cells and suppress carcinogenesis in animals, which makes them a precious commodity in the medical cannabis industry.

Terpenes have many uses:

In clinical studies, a large number of terpenoids have been proven to be toxic to tumor cells and a cancer preventive as well as anticancer efficacy. They are active in the chemoprevention and treatment of liver tumors. The U.S. medical industry is looking at using these natural compounds in the chemoprevention and therapy of human liver cancer

 

Terpene Testing in Cannabis Cultivation

It is vital for cannabis cultivators to perform in-house testing. It ensures quality product. You want to know the levels of terpenes, CBG, THC, etc., in your clients products.

Common Terpenes found in Cannabis include:

  • Nerolidol
  • β-Pinene
  • p-Cymene
  • Isopulgeol
  • Geranyl acetate
  • Fenchol
  • Pulegone
  • Menthol
  • α-Humulene
  • α-Bisabolol
  • (+)-3-Carene
  • (-) Caryophyllene Oxide
  • α-Pinene
  • Eucalyptol
  • Myrcene
  • Terpinolene
  • β-Caryophyllene
  • Limonene
  • Geraniol
  • Linalool
  • (+)Valencene
  • Camphene
  • Sabinene
  • Guaiol
  • Phytol
  • Terpineol
  • Camphor
  • Isoborneol
  • α-Terpinene
  • α-Phellandrene
  • α-Cedrene
  •  

     

    Partial list of available GC accessories

    Sample Introduction:
    Automatic liquid sampler. The optional Hewlett Packard 7673C autosampler provides great flexibility for a variety of applications.
    Headspace sampler
    Purge and trap
    Gas and liquid sampling valves

    Inlets:
    Split/splitless
    Cool on-column
    Purge Packed
    Split/splitless inlet configured for Volatiles

    Detectors:
    Electron capture
    Flame ionization
    Thermal conductivity
    Flame photometric
    Nitrogen-phosphorus

    HP and Agilent GC/MS Metallized Quadrupole

    Ions generated in the MSD source are separated in a metallized quartz quadrupole. Earlier generations of quadrupoles were composed of machined molybdenum rods held precisely in position by ceramic spacers. But the rods can become misaligned if the assembly is dropped or otherwise subjected to excessive shock. Because the 5973 quadrupole is made of a single piece of quartz, the critical distances remain constant with use, affording many years of high-precision analyses. The gold coating on the quadrupole is a very smooth surface that provides uniform fields. The state-of-the-art manufacturing processes used in the production of this quadrupole yield greater sensitivity, long-life cleanliness, and greater repeatability from instrument to instrument. The source, lens, and quad assembly is designed for easy maintenance. All parts that need cleaning are easily accessible. The variable-voltage entrance lens on the 5973N allows ions to pass through the fringing fields at the main entrance to the quadrupoles without using pre-filters. The entrance lens is cleaned along with the all the other lenses. Among the tens of thousands of MSDs in use, very few quadrupoles have had to be cleaned, or even more seldom, replaced.

    HP and Agilent High Energy Dynode-Electron Multiplier Detector

    The HP 5973 uses an off-axis high-energy-dynode electron multiplier (HED-EM) detector to reduce noise from neutrals. The HED-EM offers better sensitivity to higher-mass ions than do photomultiplier systems or EM detectors without the HED. This additional sensitivity to high-mass ions is valuable for qualitative and quantitative analyses of molecules that are easily fragmented.

    HP and Agilent GC/MS Autotuning

    Mass spectrometers must be tuned to ensure that peak shapes, isotope ratios, and mass assignments are correct. In the past, tuning was a tedious, manual process that required an expert user. Hewlett-Packard developed automatic MS tuning to make the technique available to even occasional users. Autotuning is available for PCI and NCI as well as EI operation. Additionally, there are specialized tunes for maximum sensitivity, for user-set target ratios, and for meeting the DFTPP/BFB tuning criteria for U. S. Environmental Protection Agency (EPA) methods in EI.

    Classic Spectra of the HP and Agilent Mass Selective Detectors

    It is critical that spectra be comparable between instruments and laboratories, not just that they be library searchable. Sophisticated library-search algorithms can perform matches even when there are extraneous or missing peaks. The 5973N produces classic spectra that have isotope ratios representative of the natural abundances. The spectral integrity is not compromised by artifacts from ion-molecule reactions or spectral changes due to concentration changes. The MSD has been the most popular GC/MS used in environmental, drugs-of-abuse, and sports doping testing where results must stand up to the most rigorous scientific and legal scrutiny. It has been the predominant instrument used to obtain and confirm spectra in spectral libraries.

    HP and Agilent GC/MS Sensitivity

    Sensitivity is typically specified as the signal-to-noise ratio for an injection of a pure standard in a solvent. Many factors determine the sensitivity for your specific application, including system cleanliness, the compound that is being measured, and the matrix. In a survey of HP 5973 users representing various applications and expertise level, only 16 responses out of 497 indicated that EI sensitivity is inadequate for some samples. The same survey showed high degrees of satisfaction for both PCI and NCI sensitivity.

    HP and Agilent GCMS System Inertness and Temperature Control

    A GC/MS should analyze the sample without degrading analytes because of active or hot surfaces. The 368/386 ion ratio in the cholesterol spectrum has been the classic test. In a good system, the ratio of 368/386 should be less than 50%. Figure 4 shows the spectra of cholesterol at 200°C and 250°C. At the lower temperature, the ratio is 45%, thus passing the test. At the higher temperature, the ratio is 56%, thus failing the test. Without the ability to independently control the temperature of the column oven, the GC/MS interface, and the ion source, you may not be able to optimize conditions for your analytes. The MSD allows you to set these temperatures to ensure the best performance.

    HP and Agilent GCMS Retention Time Locking

    The advanced electronic pneumatics control on the 6890 and 6850 GCs has led to the development of retention-time locking (RTL). RTL allows the matching of retention times from instrument to instrument and from laboratory to laboratory. Constant retention times benefit the laboratory in several ways. For a single instrument, it means that the retention times can be kept within narrow windows. Retention times in quantitation databases can be maintained even after the front part of the GC column is clipped in normal maintenance. For laboratories with multiple GC/MSDs, a master method can be developed and used for all instruments, simplifying initial setup and subsequent data review. Retention time locking allows easy matching of compounds detected on non-MS detectors. Peaks found by a conventional GC detector can be confidently matched with those on a MS..

    HP and Agilent GC/MS Fast Chromatography

    Faster chromatography requires makes more stringent demands on the GC/MS system. In order to effectively use smaller bore columns, the GC injection system must be able to inject small volumes, rapidly and reproducibly. The 7683 autosampler can inject 0.1 μl of extract and can support a large split ratio of 7500:1. Although the standard 6890 performs fast and reproducible temperature ramps, even greater capabilities are available on the high-voltage model. To perform fast chromatography, the mass spectrometer must scan faster but still provide good quality data. It is possible to specify a fast scan rate and still collect data that display changes in the ion ratios or losses in isotope peaks. The HP 6890/5973 system performance is shown in reducing a 17 minute analysis to less than 4 minutes while maintaining spectral integrity.

    HP and Agilent GC/MS Easy Venting and Column Changes

    The HP 5973 was designed for easy maintenance. Instrument venting can be accomplished through the MSD ChemStation Data System or the Local Control Panel. You remove one nut from inside the GC oven to remove the old column and install the new one. You can change a column without moving the instrument or removing the ion source.

    HP and Agilent GCMS Analyzer Swing-Out Design for Easy Access to the Source, Lenses, and Detector

    Experience with earlier MSDs has allowed us to improve accessibility to various components. On the HP 5973, the parts that are typically serviced are mounted on a metal plate that swings out from the left side. There is no need to remove components from the back or top of the instrument or to separate the GC and MS. The 19-part source and lens assembly is removed from the mounting plate by unscrewing two thumbscrews. Detector service is just as easy. You only need to squeeze a spring clamp to remove or reinstall the electron multiplier horn. No extra alignment is necessary. The procedure is easy and rapid. System venting is minimized with the dual filament design.

    Complete HP and Agilent GCMS Instrument Control and Data Storage

    The MSD Productivity ChemStation Data System provides integrated control of the 7673C autosampler, 7683 autosampler, 6890 gas chromatograph, and 5973 mass spectrometer. You get the full capabilities of all components, not just the basic functions. System methods store parameters for instrument control, data acquisition, data processing, and custom reporting. You can set up special displays to monitor such values as temperatures at various points and foreline pressure. Tune values are automatically saved so that long-term trends may be evaluated. This complete information storage helps with the GLP and ISO compliance.

    Hewlett Packard Chemstation Application Based on Standard Windows XP and Win 7 Environments

    The ChemStation software is a 32-bit Windows XP application that runs on a personal computer. Since most laboratory personnel are familiar with the graphical user interface, the application is easy to learn. The application software provides instrument control, data processing, and reporting capabilities.

    Flexible HP Chemstation Software for Processing, Reviewing, and Reporting Data

    The MS ChemStation Data System allows you to define complex methods that are easy to run. Data can be collected in either the scan or selected ion monitoring (SIM) mode. Method developers can set 50 groups of 30 ions each in SIM acquisition with independent dwell times, adjust ionization energies or currents, and time program events. Menus and prompts guide the user through the quantitation setup. The software comes complete with common general reports, as well as industry-specific ones.

    Ruggedness of the HP and Agilent MSD

    The GC/MSD is designed for less-than-optimal transportation and operating conditions. The instrument operates between 15°C and 35°C with the relative humidity between 25% and 50%. MSDs have been successfully used in mobile laboratories for critical tasks such as environmental and chemical-warfareagent analyses. A broad environmental operating range means that a GC/MSD can operate reliably in many facilities without modifications. Although the instrument has been designed to run under adverse environmental conditions, it has no requirements for extraordinary support utilities. The electronic autosampler does not require air for actuation; the vacuum pumps do not require watercooling; and the GC oven can operate at 5°C above ambient temperature without requiring cryogenic cooling. Both GCs offer rapid heating and cooling rates, as well as precise temperature control. The EPC even compensates for temperature and pressure changes in the laboratory. HP Technologies designs instruments that provide great flexibility. The linear dynamic range of an analysis is dependent on a large number of factors including the analyte chemistry, system inertness, and other factors. However, the 5973 MSD electronics support a total dynamic range of six orders of magnitude. To accommodate other factors that influence the linear dynamic range, the software provides various curve-fitting routines. The 5973 may well operate beyond the limits of your analyses.

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    Click on the links below to download manuals and guides for the HP 5973 MSD, 6890 GC and accessories

    Hewlett Packard 5973 Site Preparation Guide

    Hewlett Packard 6890 Site Preparation and Installation Manual

    Hewlett Packard 5973 inert Mass Selective Detector Manual

    Hewlett Packard 5973N Mass Selective Detector User Manual

    HP 5973A Hardware Manual

    Hewlett Packard Technologies 6890 Series Gas Chromatograph Service Manual

    Hewlett Packard ECD Electron Capture Detectors user guide

    Hewlett Packard FID Flame Ionization Detector user guide

    Hewlett Packard FPD Flame Photometric Detectors on 6890 GC Manual

    Hewlett Packard NPD Nitrogen-Phosphorus Detector on 6890 GC Manual

    Hewlett Packard Split/Splitless Inlet, Electronic Pneumatics Control on 6890 GC

    Hewlett Packard Purged Packed Inlet, Electronic Pneumatics Control on 6890 GC

    Hewlett Packard 7683 Automatic Liquid Sampler

    Hewlett Packard ChemStation for GC, LC, LC/MSD, CE, and A/D Systems

    Hewlett Packard G1701DA MSD ChemStation User Manual